FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n02_1437.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n02_1437.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10564894
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4977784.711623230673209Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2045601.9362238750336727No Hit
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT231060.21870545979921804Illumina Single End PCR Primer 1 (100% over 39bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC198070.18747940111845893Illumina Single End PCR Primer 1 (100% over 40bp)
ACACTCTTTCCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC117210.11094290203006296Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC111570.1056044670206819Illumina Single End PCR Primer 1 (100% over 40bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC106930.10121256304133291Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG157200.060.30389818
GATCGAG95950.058.42228
AGATAGA373800.058.3051767
GAGCACA244600.057.87973816
AGAGCAC248200.057.61934715
AGCACAC178500.057.1893317
CACACGA80200.057.09242619
ACACTCT111750.056.764861
CAGATAG147050.055.6588636
GATAGAT393800.055.3382538
ACACGAG82600.055.1782420
CACACGT89650.055.0181319
CACGTAG67000.053.91720621
ACACGTA67800.053.17952320
CACTCTT118850.053.0769232
AAGAGCA371200.052.8591714
AGATCGA110350.052.3269167
GTGACTG49150.051.496851
CACGAGA89250.051.02600521
ATCGAGA110650.051.0054249
Produced by FastQC (version 0.11.5)