FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n02_1434.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n02_1434.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11878980
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4789494.031903412582562Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2121351.785801474537376No Hit
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT175780.14797566794455416Illumina Single End PCR Primer 1 (100% over 39bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC152130.12806655116853466Illumina Single End PCR Primer 1 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG131100.059.059818
GATCGAG86350.056.0378538
AGATAGA326550.055.6874737
GAGCACA205850.054.72282416
AGAGCAC209350.054.47701315
CACACGT76950.053.5394319
AGCACAC154250.053.4204217
ACACTCT93800.053.140561
CACACGA69000.052.7074919
GATAGAT344800.052.6726048
ACACGAG71400.050.93409320
CAGATAG128050.049.9730266
CACGTAG60050.049.83508721
AAGAGCA315700.049.7215214
ACACGTA61600.048.2980420
AGATCGA102450.048.160167
ATCGAGA101850.047.6416479
CACTCTT105800.047.4535832
CACGAGA78300.046.6681821
ATAGATC409650.044.9272239
Produced by FastQC (version 0.11.5)