FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n02_1432.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n02_1432.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9939138
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3189163.2086887212955486Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2427122.441982393241748No Hit
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT115300.11600603593591315Illumina Single End PCR Primer 1 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG108950.058.82736218
GAGCACA167700.055.94249716
AGAGCAC170100.055.79115715
AGCACAC128450.054.25914417
CACACGT62850.052.3526519
CACACGA60150.052.20028719
ACACGAG60700.051.6106720
AGATAGA213850.050.9600877
CACGTAG50800.050.91842321
ACACTCT70100.050.8832741
GATCGAG52400.050.8495988
GATAGAT225100.048.1881338
CACGAGA65850.047.6794121
ACACGTA54450.046.99207320
AAGAGCA268500.046.7171514
AAGGGGG433150.045.48253667
AGGGGGG447450.044.9904868
AAAGGGG438650.044.95213366
CAGATAG85150.044.6723756
CACTCTT81300.043.8485032
Produced by FastQC (version 0.11.5)