FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n02_1421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n02_1421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8085429
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1048101.296282485443877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG14000.033.7518318
CACACGT13500.028.51953919
AGATCGG31450.026.8344297
GATCGGA33050.025.3198178
AGAGCAC29200.023.37571315
AGCACAC23550.022.88956617
GAGCACA28050.022.46222516
GGTCGCC13700.021.96669444
GTCGCCG14600.020.85239245
CGGGGTG9850.020.3127381
CCGGGTG6800.020.1319351
TCGGAAG41250.020.11267310
CGCCGTA14450.020.10079247
CGGGTTA8650.018.6668721
CTCGGTG15350.018.00798838
ATCGGAA46350.017.7500049
GGGGGTA15000.017.5509191
CGGGGTT12100.017.405871
TCGCCGT17050.017.03535846
ACACGTC12700.016.81162320
Produced by FastQC (version 0.11.5)