HJWCGAFXY_n02_1409.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HJWCGAFXY_n02_1409.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8670045
Sequences flagged as poor quality	0
Sequence length	76
%GC	41

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	533902	6.15800725371091	Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	188055	2.169019883979841	No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	13358	0.15407071128235206	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC	11885	0.1370811800861472	Illumina Single End PCR Primer 1 (98% over 50bp)
AGTTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	9114	0.10512056165798449	Illumina Single End PCR Primer 1 (98% over 50bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	8709	0.10044930562644139	Illumina Single End PCR Primer 1 (100% over 49bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCACACG	8935	57.04256	18
GAGCACA	13430	54.528725	16
AGAGCAC	13730	54.409046	15
AGCACAC	10015	53.93301	17
CACACGA	5765	53.250042	19
ACACGAG	5865	51.684402	20
AAGGGGG	69430	48.79518	67
AAAGGGG	69735	48.601833	66
CACGAGA	6275	48.362637	21
CACACGT	4115	48.0616	19
AGGGGGG	73030	47.72592	68
GGTCGGA	2570	46.82139	2
AAGAGCA	22045	45.692528	14
GTATCAT	102955	44.19829	51
CATTAAA	106990	43.954903	55
AAAAGGG	78040	43.667248	65
ATCATTA	105955	43.61099	53
CCGTATC	106250	43.605682	49
CACGTAG	3235	43.604908	21
CGTATCA	106695	43.593872	50