FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n02_1215b-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n02_1215b-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8224464
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1467621.7844567135317266No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC493760.6003552328759661Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG21350.043.2861418
AAGGGGG88400.034.31445767
AGGGGGG100250.032.3870468
AAAGGGG101700.029.37969266
GGGGGTA25400.028.3346311
CACACGT17750.028.20118119
GATCGAG16700.027.2565068
GCGGTCC5800.026.6159042
GGCGGTC8700.026.2295911
GCGGCGC8250.025.9581241
GAGCACA51200.025.91392716
CACACGA16150.025.79312919
CGGCGCT12950.025.7543891
ATAGATC72550.025.7213339
AGAGCAC52600.025.55744215
CACGTAG15000.025.43492721
GATAGAT72950.025.3906428
AGATAGA72200.025.2687347
CGGGGTG11150.024.8742281
ACACGAG17250.023.53842420
Produced by FastQC (version 0.11.5)