FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8835080
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA637960.722076087596264TruSeq Adapter, Index 14 (97% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG405980.45950913857033554No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG17600.043.7635351
TGACTGG19050.040.581292
GACGTGT94750.036.137821
ACTGGAG23600.033.4979064
ACGTGGG13700.030.3790257
GAGTTGG13650.025.8781788
ACGTGTG135650.025.2201462
TGGAGTT31400.024.8425626
GGAGTTC14350.024.6159677
AAAAGGG54650.024.03397470
GACGTGG17650.023.7825136
GGAGTTG16550.023.0343487
TCACAGA227600.022.84615731
TCCGCTC17700.022.32826813
AAAGGGG26750.022.12826570
CACAGAT242800.021.96393632
GTCACAG236300.021.54589730
GGGTGGC25200.021.12649770
ACAGATC256300.020.71140533
CGTGGGG21050.020.4360988
Produced by FastQC (version 0.11.5)