FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n01_POLa-5-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n01_POLa-5-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10801248
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG126380.11700499794097868TruSeq Adapter, Index 1 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC72650.013.58789737
CCCCGGG11600.013.574837
TATGCCG73600.013.45514947
ATGCCGT77550.012.90518248
GATCTCG74200.012.87945640
GCCGTCT68250.012.71536850
TGCCGTC75800.012.695312549
TGCCCCG13000.012.3830765
CGATCTC77750.012.33641239
GTATGCC83200.012.112926546
CCCGGGT13650.012.0488368
CGTGTGC24750.012.0245531
ACGATCT82150.011.80350438
CTCGTAT86350.011.31046943
TCACGAT90900.011.27933936
GTGCCCC15400.011.134984
CCGTCTT79350.011.11593751
GCACACG109950.011.07566812
CACACGT110500.011.05220813
CAGTCAC106050.010.95563228
Produced by FastQC (version 0.11.5)