FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n01_POLa-05-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n01_POLa-05-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12157193
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG32750.013.78417847
TATCGCG32600.013.20695140
GCGTGGA21250.012.84871460
GCACACG108150.012.45771912
CCGATGT104650.012.20847934
CACACGT111250.012.17349313
CGTGTGC29000.012.0733011
ACACGTC112600.011.96548314
GTCACCG111550.011.86133930
ACGTCTG116300.011.85567116
GTGCGTG14950.011.70676158
ACCGATG111250.011.70444533
ATGCCGG23350.011.68993348
CCCCGGG14700.011.66476257
CGATGTA110450.011.662456535
GCCGGCG15050.011.62615350
TCACCGA116850.011.38315831
CAGTCAC118100.011.37870428
CACGTCT119750.011.367990515
AGCACAC119850.011.35836611
Produced by FastQC (version 0.11.5)