FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n01_POLa-04-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n01_POLa-04-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7169981
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGGA17150.018.36986460
CGTGTGC27450.017.6025831
ACGTGTG18950.016.9987981
GTGCGTG11250.016.49068858
TATGCCG31900.015.24840547
GTGTGCT32500.014.8609832
AAAAGGG54050.014.57336470
GTATGCC38400.014.39879646
CATCGCG31450.014.13538440
AGGCATC87200.014.13027837
GCGTGAA17700.014.04147260
GTGTGCG11900.013.82503856
TCCGATC56250.013.6878055
GTGGGGG23900.013.47474366
ATGCCGT30900.013.36361148
CCGATCT57250.013.3264556
TCGCGGA10750.013.02494942
CTGCGTG14900.012.92087458
CATCTCG36000.012.83500340
TAGGCAT115750.012.72794836
Produced by FastQC (version 0.11.5)