FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n01_POLa-03-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n01_POLa-03-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10248494
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG291230.28416858125691447TruSeq Adapter, Index 4 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG28900.017.0823541
AAAAGGG82050.015.61606770
CGTGTGC38350.015.4293561
TATGCCG148700.014.73254747
GCCGTCT119600.014.71808550
TGCCGTC140100.014.71267549
GCTTGAA117150.014.64097560
ATGCCGT144750.014.62686648
CTGCTTG110250.014.5089858
TGCTTGA110650.014.45709459
CCGTCTT129800.013.80630951
GTATGCC161200.013.633553546
GTGTGCT44250.013.3668872
CTCGTAT161750.012.98424843
CGTATGC171200.012.85764445
ATCTCGT168000.012.81367141
AATCTCG169500.012.72092740
TCTCGTA170900.012.55533442
ACTGACC200250.012.28802533
GCGTGAA29100.012.14911360
Produced by FastQC (version 0.11.5)