FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n01_POLa-02-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n01_POLa-02-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10779679
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGGA19650.012.4701960
ACGTGTG21950.012.1220791
GTCACAC96150.011.32240330
CACACAG102150.010.89723432
GAACTCC116100.010.82103622
ACACGTC118800.010.51584814
ACGTCTG121050.010.49383816
CACACGT120650.010.44156613
GCACACG120700.010.35026512
CAGTCAC109100.010.29681828
TATGCCG38900.010.25523947
CCCCGGG9600.010.2064647
AGTCACA109600.010.18843929
ATGCCGG23400.010.16912548
TCCAGTC112650.010.15891626
GCCGGGG12450.010.11867550
CACAGTG110600.010.09631934
CACGTCT124600.010.08251715
CCAGTCA111950.010.00342327
ACACAGT110700.09.99233433
Produced by FastQC (version 0.11.5)