FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n01_POLa-01-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n01_POLa-01-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14821553
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG780290.5264563031957582TruSeq Adapter, Index 6 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG161600.024.30671170
GCGTGAA73200.022.14087360
TGCGTGA60700.019.95318659
GCCGTCT227450.019.9090350
CTGCGTG62800.019.73101458
ACGTGTG42100.019.5445821
ATGCCGT304600.019.4733948
CTGCTTG196050.019.3181858
TATGCCG328650.019.26223647
TGCCGTC292950.019.08908349
CCGTCTT244150.018.86645551
GTATGCC347550.018.56715646
TGCTTGA207250.018.51146959
GCTTGAA225750.018.29696760
AAAGGGG53800.018.08994570
CTCGTAT349400.017.932643
CGTGAAA94650.017.71506361
CGTATGC361200.017.71047645
TATCTCG375150.017.69076240
TCTCGTA367950.017.46614342
Produced by FastQC (version 0.11.5)