FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n01_POLa-00-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n01_POLa-00-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9582921
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC30150.014.7506571
ACGTGTG23800.014.272161
CATCGCG26450.013.49843940
CCGGCGT12850.012.25719851
ATCGCGG14200.011.83210241
GCCGGCG11900.011.46903750
TGCCGGC17000.011.11614349
GACGTGT19250.011.09670451
GTGTGCT40600.011.0342582
CGGCGTG13150.010.91278152
TATGCCG22950.010.36896847
CAGTCAC107200.010.34879228
AGTCACC106750.010.26315629
GCCGGGG11300.09.91264660
TGCCGGG14250.09.82559159
ATCGCGT19950.09.82548941
CCAGTCA115450.09.79115127
TCCAGTC118350.09.7584326
GCGTGGA17600.09.745440560
TGTGCTC48300.09.7097953
Produced by FastQC (version 0.11.5)