FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n01_1437.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n01_1437.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10564894
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATG1331421.2602303440053446TruSeq Adapter, Index 19 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGCGTATG745610.7057430013022374TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGCGGATG371120.3512765958655146TruSeq Adapter, Index 19 (97% over 42bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAA273690.2590560776094867TruSeq Adapter, Index 19 (100% over 38bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAA230370.2180523533884959TruSeq Adapter, Index 19 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGGGGATG198860.18822716063218428TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGGATG187630.17759761716492375TruSeq Adapter, Index 19 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGGGGTTT185780.17584653475936435TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGCGTTTG145400.137625611766668TruSeq Adapter, Index 19 (97% over 42bp)
GGGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGA144120.13641405204822687TruSeq Adapter, Index 19 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGCGGTTG142200.13459671247056526TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATT137880.13050769842082655TruSeq Adapter, Index 19 (97% over 48bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAA128610.12173335577242897TruSeq Adapter, Index 19 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTTTG123040.11646117793515014TruSeq Adapter, Index 19 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGGGTATG112870.10683495735972362TruSeq Adapter, Index 19 (97% over 42bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG421100.051.03837270
GACGTGT40350.045.2948041
GTGGAAA367350.043.32872462
ACGTGTG98150.041.522561
TGGAAAA447550.039.0135863
CGTGGAA220300.038.61203861
GGAAAAA524900.036.19880364
AAAGGGG76650.034.62114770
AAAAAGG641300.034.40887569
TGCGTGA168500.033.07288459
GGTGGAA228850.032.47356861
CTGCGTG207350.030.97736558
AAAAAAG804250.030.0625168
GCGTGAA232600.030.03874460
GCGTGGA282200.029.82005160
GTCTGCG118250.029.36452356
CGTCTGC148000.028.8291555
TGCGTGG183500.028.82420359
TCTGCGT235900.028.41536757
GTGCGTG166600.027.54483258
Produced by FastQC (version 0.11.5)