FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n01_1434.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n01_1434.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11878980
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATG560870.472153333030277TruSeq Adapter, Index 20 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGCGTATG378240.3184111767171929TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGTATG344940.29037846683806184TruSeq Adapter, Index 20 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGCGGATG337920.28446886853921804TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGTCGTATG302880.2549713864321684TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGGGGTTG226860.19097599288827827TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGCGGTTG219890.1851084857454091TruSeq Adapter, Index 20 (97% over 41bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGC212590.17896317697310712TruSeq Adapter, Index 20 (100% over 38bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCC180620.15205009184290233TruSeq Adapter, Index 20 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGGATG178530.15029068152316108TruSeq Adapter, Index 20 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGCGTTTG124740.10500901592560977TruSeq Adapter, Index 20 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG432950.049.6375570
GTGGAAA360250.043.3253362
CGTGGAA196200.041.42586561
GACGTGT38550.040.9604531
ACGTGTG87200.040.1911281
AAAGGGG85650.037.2341570
TGGAAAA458150.036.9705863
GCGTGGA224600.035.2837760
TGCGTGA121500.035.0320659
TGCGTGG141650.034.9414759
GTCTGCG85100.034.34412456
GTGCGTG131300.033.98920458
CTGCGTG147000.033.9069158
GGAAAAA567050.033.38287464
AAAAAGG665000.033.1115469
GGTGGAA232800.032.417161
GTGTGCG120900.032.36764556
CGTGAAA176850.031.96508261
GCGTGAA174350.031.68060360
TGTGCGT157250.031.40739657
Produced by FastQC (version 0.11.5)