FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n01_1432.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n01_1432.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9939138
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATG3384773.4054965329991393TruSeq Adapter, Index 21 (97% over 49bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTC140310.1411691838869729TruSeq Adapter, Index 21 (100% over 38bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCG114370.11507034111006409TruSeq Adapter, Index 21 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATGTCGTATG100020.10063246933486585TruSeq Adapter, Index 21 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG68950.042.7080841
AAAAGGG350300.041.35423770
AAAGGGG110500.040.80592770
GACGTGT27850.034.5744931
TGAAAAA802150.033.9497763
AAGGGGG39250.033.17973770
TTGAAAA749100.032.5737862
GAAAAAA851050.032.23351364
CTTGAAA752800.032.03239461
AAAAAGG473600.031.1346669
GCTTGAA865000.027.69534360
CGTGTGC135200.027.063751
GTGTGCT136950.026.7066252
GCGTGAA93450.026.59450360
CGTGAAA97700.026.11835361
TGCTTGA903700.025.47887259
AAAAAAG619650.025.22299268
TGCGTGA74450.024.96551159
CTGCTTG954500.024.05977858
TCTGCTT1022250.022.59532457
Produced by FastQC (version 0.11.5)