FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n01_1421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n01_1421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8085429
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG16950.017.55305134
GCACACG18450.017.26032812
CGTACGA17700.016.41376535
CACACGT19900.015.65095813
ACACGTC21300.015.11523514
AGATCGG21950.014.0374611
GATCGGA23000.013.5428212
AGCACAC25150.013.07958211
ACGTCTG23450.012.98332116
GCCGTCT19750.012.75753450
TGCCGTC22500.012.44253349
CACGTCT25450.012.10045115
GATCTCG22750.011.847630540
CAGTCAC27250.011.55835728
GTATGCC24850.011.54752246
GTCACCG27000.011.27876630
CGATCTC23800.011.17786439
ACCGTAC27750.010.973865533
GAGCACA29400.010.95076710
TATGCCG27000.010.75760647
Produced by FastQC (version 0.11.5)