FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n01_1409.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n01_1409.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8670045
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATG1216361.4029454287722845TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGCGTATG1015921.1717586240901863TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGCGGATG555280.6404580368383325TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGGATG195730.225754306926896TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGGGTATG177460.2046817519401572TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG176810.2039320441820083No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGCGTTTG127470.14702345835575248TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATT112080.12927268543588874TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGCGTATT97350.11228315423968388TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGGGTTTG92540.10673531682938207TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGGA180800.054.24643360
AAAAGGG509800.053.15772270
CTGCGTG135150.053.0926458
TGCGTGG112550.052.68286559
GTGCGTG103100.051.60376458
TGCGTGA122100.051.40035659
GTCTGCG80950.050.89277356
CGTGGAA194950.050.73999861
GTGTGCG83200.050.5682256
GTGGAAA377150.049.90308862
GCGTGAA160600.048.49377460
TCTGCGT155800.048.1674757
GGGTGGA182800.048.02339660
GCCGTCG106150.047.96885350
CGTCTGC102000.047.354455
TGCCGTC243750.047.27847749
TCGCGGA106850.047.1720242
ATGCCGT259900.046.4950448
CGCGGAT107950.046.36709243
TGTGCGT123550.045.9520257
Produced by FastQC (version 0.11.5)