FastQCFastQC Report
Tue 2 Apr 2019
HJWCGAFXY_n01_1215b-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJWCGAFXY_n01_1215b-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8224464
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG210350.2557613480951464TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGGA31050.026.38089860
CTGCGTG31000.024.38998458
AAAAGGG103200.023.9173770
TGCGTGA26850.023.59769659
ACGTGTG19000.023.0367451
GCGTGAA36600.022.95437660
TGCGTGG23350.022.93717259
TGCCGTC74250.022.48081249
GTGCGTG18100.021.85341658
GCCGTCT45750.021.41691850
TATGCCG92500.021.41237347
ATGCCGT79200.021.29668248
GTGGAAA77200.021.03952662
GTCTGCG18900.020.74319656
GCCGTCG32950.020.70948250
CGTGGAA41300.020.17261761
GTATGCC104500.020.058646
TCGCGTA32350.020.0181942
CGTATGC102950.019.85073545
GGGTGGA36400.019.71459260
Produced by FastQC (version 0.11.5)