FastQCFastQC Report
Fri 12 Apr 2019
HJW27AFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJW27AFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7870100
Sequences flagged as poor quality0
Sequence length41
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7736469.830192754856991No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAAA365030.46381875706788983No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAA337090.42831730219438124No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAAA318330.404480248027344No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAAA304550.3869709406487846No Hit
GATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAA239140.30385890903546336No Hit
TACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAAAAA218280.27735352790942935No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAAAA182360.23171243059173324No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAAA178460.2267569662393108No Hit
TACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAAAAA177600.22566422281800738No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAAAA167620.21298331660334685No Hit
TACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAAAA163330.20753230581568213No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAGT155380.19743078232805172No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAAAA147700.1876723294494352No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCG146320.1859188574478088No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN144140.18314887993799317No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAA137300.17445775784297532No Hit
TACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAAAGT134640.17107787702824614No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAGT129890.16504237557337262No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAA120800.1534923317365726No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAAGT115590.14687233961449028No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAAA113260.14391176732188918No Hit
TACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAAAAA109710.1394010241292995No Hit
TACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAAAAA108050.13729177519980687No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAAAA101430.12888019211954105No Hit
TACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAAAGT100650.12788909924905656No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAGA95740.12165029669254519No Hit
GGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGG93840.1192360961105958No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAAT91130.11579268370160481No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAA88310.11220950178523781No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCATA87420.11107863940737729No Hit
TACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAAGT85640.10881691465165627No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAATT84920.10790205969428597No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCATA82970.10542432751807473No Hit
GGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80580.10238751731235943No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAGA80390.1021460972541645No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAAAA79150.1005705137164712No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAGT78830.10016391151319551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGGA93500.034.4823326
ACACGGG88300.034.39292525
CACACGG88600.034.3361424
ACGGGAA89850.034.22838627
CGGGAAT93550.034.03380228
CAATTTG32100.033.89732725
GGGAATT95100.033.84678329
GCACACG90150.033.5704923
GCCCACA125250.033.5484623
TCAAAGT142750.033.490735
CCCACAA128050.033.44394324
CTACATG57750.033.4423825
CGATACT159950.033.41161
CATGAAT157150.033.4067728
ATTCCGG25650.033.35788312
GGGACCT10400.033.31453311
ACCGGGC15900.033.232214
CAAGAAT125000.033.0421928
CCACAAG127350.032.99545725
AGCACAC94100.032.960722
Produced by FastQC (version 0.11.5)