FastQCFastQC Report
Fri 12 Apr 2019
HJW27AFXY_n02_CCPD53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJW27AFXY_n02_CCPD53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11833382
Sequences flagged as poor quality0
Sequence length41
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAA152472812.884972360395363No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAA131702511.129742959366984No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAA9444307.981065768011207No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAT3183012.689856543125203No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCTA3068792.5933329964333103No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAT2838882.399043654637364No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCTA2645532.2356499604255147No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAT2281431.9279610850051154No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCTA2114121.786572934094412No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCCA1625341.3735211117159913No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCTT1522771.286842594957215No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCCA1497861.2657919773062343No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCGA1472651.2444878395711387No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCGA1439231.216245702200774No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCTT1437521.214800637721321No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCTT1322931.1179644162590203No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCGA1200851.0147986433633258No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCGT956330.8081628734709992No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCGT929140.7851855031807474No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCCA927240.783579875981355No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCGT788970.6667324692129435No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCCT740560.6258227783063202No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCCT737830.6235157455408775No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAG677300.572363843236025No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAG595220.5030007482222749No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCGCCCCGAATTCAA576500.48718109497352485No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAG553080.4673896270736464No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCCT547930.46303753229634603No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTAAA453830.38351673257907165No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAAGTCAA382950.323618387372266No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCGACCCGAAGTCAA378110.3195282633485507No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAC334470.2826495417793493No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAC326630.2760242169144882No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTGTT315540.2666524244717191No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAAGTCAA287710.24313421133535623No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTAAA240330.20309493938419299No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTGTT238340.2014132561595662No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTAAA229350.1938161043055992No Hit
AGATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAA226150.19111189007504364No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAC200120.16911479744336827No Hit
AGATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAA197670.16704438342309916No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTGTT196150.16575988166358527No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTGAA193040.16313172345826407No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTGAA171460.14489517874095503No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTGAA167380.1414473055969967No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTGAT158590.1340191671324394No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGCCGCCGCATTCAA148460.12545863895883697No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAAGTCTA143060.12089527744477445No Hit
CGATCCTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAA142170.12014316786190118No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCGACCCGAAATCAA141300.11940795961796889No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTGAT138330.11689811078523453No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCGACCCGAAGTCTA136060.11497980881543417No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCGACCCGCATTCAA134370.11355164567492201No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTAAT130010.10986715378579007No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTGAT129840.10972349240479179No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCGACCAGAATTCAA128790.10883617211039076No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTGTA127100.1074080089698786No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAAGTCTA125210.10581083243995672No Hit
AGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAA121510.10268408473587687No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGCCGCAGAATTCAA120790.10207563653400187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCT5800.035.0577741
ACAGGCT358.5973807E-735.0577741
GGAGGCT700.035.0577741
CTATGCT2550.035.057771
GGATCGT301.20382265E-535.057771
CGATTCT8550.035.057771
TACGGCT251.6949323E-435.057771
AGAGTCT5650.035.057771
AGATTCT1050.035.057771
CTATTCT454.44561E-935.057771
AGAGGCT8050.035.057771
CGAGTCT16250.035.0577661
CGATACT10055300.035.056031
CAGCGTC251.6977631E-435.047822
GACACAC358.616498E-735.047822
CCTACTG552.3646862E-1135.047822
GTTAGTC200.002404183135.047822
GAGAGTC1200.035.047822
TATGCTG1650.035.047822
TATGCTC4550.035.047822
Produced by FastQC (version 0.11.5)