FastQCFastQC Report
Fri 12 Apr 2019
HJW27AFXY_n02_CCPD52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJW27AFXY_n02_CCPD52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25098996
Sequences flagged as poor quality0
Sequence length41
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAA318811712.70216944135933No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAAA287405011.450856440632128No Hit
GATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAA22006588.767912469486827No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCATA8190053.2630986514360973No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCATA7802743.1087857060099138No Hit
GATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCATA5779402.3026419064730717No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAT5519792.199207490211959No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAAT5197612.070843789926896No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAGA4752401.8934621926709738No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAGA4221371.6818879926511803No Hit
GATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAT3999781.5936015926692846No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCATT3679481.4659869263296428No Hit
GATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAGA3456541.3771626562273647No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCATT3454991.3765451016447032No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCACA3351691.3353880768776567No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAGT3098491.2345075476325826No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCACA2866321.1420058395961337No Hit
GATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCATT2770381.1037812030409504No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAGT2655391.057966621453703No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAG2404790.9581219902182541No Hit
GATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAGT2363660.9417348805506005No Hit
GATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCACA2307990.9195547104752715No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAAG1938270.7722500135065163No Hit
GATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAG1855980.7394638415018673No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCACT1542310.6144907150867708No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCACT1329910.5298658161465901No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAC1317810.5250449061787172No Hit
GATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCACT1128360.44956379928503915No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAAC1050380.4184948274425001No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGCCCCGAATTCAAA1008680.4018806170573516No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCTTT926150.3689988236979678No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCTTT922330.36747685046844103No Hit
GATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAC914460.36434126687776675No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCTAA780740.3110642353981012No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCTAA745830.297155312507321No Hit
GATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCTTT696490.27749715566311894No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCTTA554330.22085743987528425No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCTTA542950.21632339397161543No Hit
GATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCTAA540790.21546280177900343No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAAGTCAAA523530.20858603268433523No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAAGTCAAA486150.1936930066844108No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCTTG479630.191095293214119No Hit
GATACTCCTTTCAAGACCTAGCTAGCCAATTAGAATTCAAA468810.18678436380483107No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCATC453510.18068850244049603No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCTAT413900.16490699468616193No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCTAT397780.15848442702648347No Hit
GATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCTTA394800.15729712853852798No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTAAAA382770.15250410813245277No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCTTG374910.14937250876489244No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCATC367950.14659948947758708No Hit
TATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAA362710.14451175656588017No Hit
GATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAAGTCAAA351210.139929899984844No Hit
GATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCTTG350480.13963905169752608No Hit
TATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAAA342700.13653932611487726No Hit
GATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCATC327960.13066658124492309No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGACTTCAAA299700.11940716672491601No Hit
GATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCTAT297510.11853462186296217No Hit
GATACTCCTTTCAAGACCTAGCTAGCCCATTAGACTTCAAA289340.11527951157886954No Hit
GATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAGC268470.10696443794006741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACTT9250.035.056731
CAAACTG552.3646862E-1135.056731
GAGTCAC750.035.056731
GAATCTC950.035.056731
TATGCTG1500.035.056731
TATGCGG552.3646862E-1135.056731
TATGCCC406.171467E-835.056731
GAAAGTC1350.035.056731
GAAAGGC200.002401240235.056731
GTTCGTC700.035.056731
GATGGTC358.599891E-735.056731
TATTCTG406.171467E-835.056731
GATATTC5850.035.056731
GATGCTG9900.035.056731
CATACTG1100.035.056731
CATACGG358.599891E-735.056731
CGTACGC200.002401240235.056731
CATCGTC1500.035.056731
TAAGCTG200.002401240235.056731
TAAGCTC552.3646862E-1135.056731
Produced by FastQC (version 0.11.5)