FastQCFastQC Report
Fri 12 Apr 2019
HJW27AFXY_n02_CCPD51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJW27AFXY_n02_CCPD51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29091731
Sequences flagged as poor quality0
Sequence length41
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAAA353855312.163432282527292No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAAA309974710.65507927321341No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAAA17402225.981844119210369No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAAA10956593.7662214049758678No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAATA6498402.233761889246123No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAGA5852022.011575041718899No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAATA5730001.9696318517450886No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAACA5352751.839955827997997No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAGA4924531.6927593617581573No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAAT4714331.6205051531653445No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAACA4620221.5881557546369447No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAAT4017401.380942234066443No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAATA3338971.1477385103004012No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAGT3152561.083661883165357No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAGA2919241.0034603991079114No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAAG2845040.9779548697188214No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAAGTCAAAA2697960.9273975481211483No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAATT2538710.8726569072153183No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAGT2520960.866555517098656No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAACA2479850.8524243538481777No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAAT2386560.8203568223561534No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAAG2335330.8027470073884568No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAAGTCAAAA2173630.7471642027763834No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAATT2156740.741358429307627No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAATA2065460.7099818157950106No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAAC2000170.687539012374341No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAACT1982250.681379186408674No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAAC1749620.6014148831501296No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAGA1743620.5993524414205534No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAACT1655630.5691067334563213No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAACA1594310.5480285789800545No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAGT1548080.5321374654536714No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAAT1419490.4879359017859748No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAAGTCAAAA1355140.4658162142362722No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAAG1345210.46240287317382384No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAATT1306160.4489798149171667No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAAGTCAAGA1249160.4293866184861945No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAAGTCAATA1081510.3717585591589583No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCATAA1032400.35487747360237865No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAAGTCAAGA1004380.34524587072525864No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCATTT982340.33766983477194945No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAAGTCAACA969680.3333180827225441No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAAC930360.31980221458805597No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAACT925610.31816944821880827No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAGT891450.30642727997175556No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAAGTCAATA888180.30530324922913665No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCATAA886470.3047154533362074No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAAG814790.28007614947353937No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCATTT809560.27827838776592567No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAAGTCAACA791300.27200169010224934No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAATT775270.26649153328139874No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAAGTCAAAA734750.2525631768009955No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAAGTCAAGT709120.24375311321282325No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAAGTCAAGA627750.21578296595688995No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCATTA620550.21330803588139874No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAAC612870.21066811046754147No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAAGTCAATA571870.19657475864877208No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAACT568790.19551603856092303No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAAGTCAAAT535260.18399042669547577No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAAGTCAAGT534290.183656998615861No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCATAA528060.18151549661998456No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCATTG526900.18111675788559986No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCATTA522300.17953555255959158No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCATTT512750.1762528328066831No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGCCCCGAATTCAAAA496800.17077017520889354No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAATC477720.16421161050884184No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACACGAATTCAAAA473320.16269915324048612No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAAGTCAACA458940.15775616789526895No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAAGTCAAAT426100.1464677368287229No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCATAT423250.1454880770071743No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCATTG421930.14503433982666758No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAAGTCAATT421790.1449862161863108No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAATC418900.1439928067532317No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAGC372890.12817731609026634No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGCCGAATTCAAAA366230.12588800577043696No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCATAT363790.12504927946707606No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAAGTCAACT360060.1237671281918563No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAAGTCAAGT352640.12121657525294731No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTTAAAA342390.11769323729825497No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAAGTCAAGA340760.1171329406283868No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAAGTCAATT338840.11647295927492249No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCATTA337560.11603297170594627No Hit
ATAATCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAAA319280.10974939923650469No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCATAA315120.108319439637332No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAAGTCAATA312040.10726071954948296No Hit
ATACTCCTTTCAAGACCTAGCTAGCGAATTCAAAAAAGCAC301450.10362050989678132No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAGC300170.1031805223278051No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTCG301.20287605E-535.0620541
ATCCTCG4400.035.0620541
TTAGGCC3150.035.0620541
ATGATCC454.440153E-935.0620541
TTGCTCC950.035.0620541
ATAATCT454.440153E-935.0620541
CATCTCC200.00239945835.0620541
TTACTCG1350.035.0620541
AGAGTCA358.589741E-735.0620541
CCACTCC552.3646862E-1135.0620541
ATAAGCG454.440153E-935.0620541
ACGCTCC358.589741E-735.0620541
TTAGTCC2450.035.0620541
TTAGTCA358.589741E-735.0620541
CGGCTCC552.3646862E-1135.0620541
TTCGGCA406.163282E-835.0620541
AGGGGCT358.589741E-735.0620541
ACACTGC301.20287605E-535.0620541
AATAGTC200.00239945835.0620541
AATAGGC200.00239945835.0620541
Produced by FastQC (version 0.11.5)