FastQCFastQC Report
Fri 12 Apr 2019
HJW27AFXY_n02_CCPD50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJW27AFXY_n02_CCPD50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52474917
Sequences flagged as poor quality0
Sequence length41
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAAAAA24793224.724775457958323No Hit
TACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAAAAA20022553.8156420523733274No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAAAA19984863.8084595731709303No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAAAA18643383.5528174346612116No Hit
TACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAAAA17833713.3985208590229883No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAAAA15712382.994264859913928No Hit
TACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAAAAA11937392.274875441918279No Hit
TACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAAAAA11863222.260741069871535No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAAAA11129572.120931415670462No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAAAA8707321.659329923285062No Hit
TACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAAAAA6595671.2569186150404013No Hit
TACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAAAA4927020.9389285932553261No Hit
TACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAAACA4824680.9194259421124954No Hit
TACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAAAGA4692470.8942310475688794No Hit
TACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAAATA4242540.8084891301495531No Hit
TACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAAACA3858740.7353494241829863No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAACA3782630.7208453516944104No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAAGA3656460.6968014832686634No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAAGA3588080.683770495530274No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAACA3540450.674693777981583No Hit
TACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAAAGA3532920.6732588066790082No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAATA3452140.6578647851887026No Hit
TACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAAATA3421720.6520677298069857No Hit
TACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAACA3381860.6444717196980035No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAATA3276550.6244030838581413No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAACA3014860.574533543330807No Hit
TACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAAGA3001870.5720580749084367No Hit
TACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAATA2989090.5696226256060586No Hit
TACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAAAAG2959300.563945627584318No Hit
TACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAAAAC2761210.5261961633974571No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAAGA2704610.5154100577233881No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAATA2605950.49660869401660984No Hit
TACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAAAAT2598180.49512798657690116No Hit
TACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAAAGT2548720.48570253098256455No Hit
TACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAAAGA2438440.4646867759695551No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAAAG2408480.4589773815173448No Hit
TACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAAACA2265450.4317205494579439No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAAAT2241870.42722697398454196No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAAAG2233290.42559190708200645No Hit
TACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAAACA2214320.4219768465760508No Hit
TACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAAAAC2196230.41852948523958594No Hit
TACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAAATA2180540.4155394852744598No Hit
TACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAAAAG2153730.4104303776221314No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAAGT2149960.4097119391346536No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAACA2149760.4096738256870421No Hit
TACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAAAGA2105710.4012793388505979No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAAAT2099560.40010735033654266No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAAAC2097540.39972240451566604No Hit
TACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAAAAT2086550.3976280705694113No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAAGA2043590.3894413020224501No Hit
TACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAAATA1999190.3809801166526857No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAAAC1977480.3768429019144518No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAAGT1958220.37317257690945943No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAATA1898970.36188146805453736No Hit
TACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAAAC1893520.3608428766071226No Hit
TACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAAAT1835260.34974042931787774No Hit
TACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAAAG1821600.3471372808460088No Hit
TACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAAAGT1801290.34326686024105574No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAAAC1739870.3315622204795483No Hit
TACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAAACT1713670.32656935884243515No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAAAG1700000.3239643046981856No Hit
TACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAAATT1684890.3210848337311329No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAACA1609670.30675036608442846No Hit
TACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAAGT1580420.3011762743712391No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAAAT1560190.2973210991453307No Hit
TACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAAAAG1504860.28677701386359505No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAAGA1483290.28266647853868926No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAATA1481720.2823672879749386No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAAGT1437710.2739804238280167No Hit
TACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAAAGT1422720.27112382092953097No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAATT1393080.26547540799349906No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAATT1392160.2653000861344859No Hit
TACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAAAAT1350700.25739916844461136No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAACT1350340.25733056423891054No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAACT1349980.2572619600332098No Hit
TACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAAAAG1325070.2525149301331911No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAAAG1302910.24829196013783117No Hit
TACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAAACT1301800.24808043050358708No Hit
TACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAAAAC1301770.24807471348644536No Hit
TACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAAATT1300320.24779839099126158No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAAAC1245090.23727336243333175No Hit
TACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAAACA1233770.2351161412985179No Hit
TACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAAAAT1233150.2349979896109221No Hit
TACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAAAGA1215330.23160208142873287No Hit
TACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAAAAC1206890.2299936939395254No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAAAT1180710.22500464364717337No Hit
TACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAAATA1153900.21989553599484493No Hit
TACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAAAGT1131660.21565732062044043No Hit
TACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAACT1125040.21439576550449807No Hit
TACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAATT1124960.21438052012545347No Hit
TACTCCTTTCAAGACCTAGCTAGCGAATTCAAAAAAGCACC1117430.21294554882287856No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAAGT1097060.20906369418364207No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAACT1008160.19212226672030752No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAATT986130.18792407046589515No Hit
TACTCCTTTCAAGACCTAGCTAGCGAATTCAAAAAAAGCAC947380.1805395899911571No Hit
TACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAACA941690.17945526240660847No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAAAT906630.17277397504030353No Hit
TACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAAATT905820.17261961557747676No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAAAC887130.1690579138981773No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAAAG882640.16820226699929797No Hit
TACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAAGA871000.16598406434830568No Hit
TACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAAACT845200.16106742960641557No Hit
TACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAATA815600.155426639359906No Hit
TACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAAACT783080.14922939277826774No Hit
TACTCCTTTCAAGACCTAGCTAGCGATAACGAATTCAAATT781970.14901786314402365No Hit
TACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAAAAG778020.1482651225536955No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAAGT777570.14817936729656953No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAACT760460.1449187618534013No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAATT750770.14307216531662165No Hit
TACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAAAAT732470.1395847848601647No Hit
TACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAAATC709890.13528177662482058No Hit
TACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAAAAC703890.13413837319647404No Hit
TACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAAAGT675540.12873579199753665No Hit
TACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAAAGC660690.12590586851237898No Hit
TACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAATAA645420.12299590678723704No Hit
TACTCCTTTCAAGACCTAGCTAGCGAATTCAAAAAAGCAGG600260.11438989031654877No Hit
TACTCCTTTCAAGACCTAGCTAGCGAATTCAAAAAAGCACG574260.1094351421270471No Hit
TACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAATTT561230.10695205101515454No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAATT559390.10660140729712826No Hit
TACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAAAG552520.10529221037167148No Hit
TACTCCTTTCAAGACCTAGCTAGCCGCCCCGAATTCAAAAA551330.10506543535838274No Hit
TACTCCTTTCAAGACCTAGCTAGCGACCATGAATTCAAATC551060.10501398220410715No Hit
TACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAAAC547200.10427839266520422No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAACT537450.1024203620941411No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAATC535530.10205447299707018No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAATC532430.10146371455909116No Hit
TACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAATTG526570.1003469905440727No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACT251.6938274E-435.061941
ATATGCT2550.035.0619351
TATGCCG200.002399514435.0619351
TTCTGCT5450.035.0619351
TACTCCT48167650.035.0536041
AGTGCTT601.8189894E-1235.052192
ACGGCTT102900.035.052192
AATCTTT552.3646862E-1135.052192
GCTACTG1200.035.052192
AGTCTCT750.035.0521852
CCTGCTG200.002402790135.0521852
GTCTCTT750.035.041543
ATCCGGT503.237801E-1035.041543
CGCTCTT251.6996314E-435.041543
ATCTTTT406.195296E-835.041543
CAGCTTT1650.035.041543
TGCTTCT200.00240974735.0315324
TCTTCTG251.702487E-435.0315324
ACGGGTC601.8189894E-1235.0315324
ACGTGTC301.210092E-535.0315324
Produced by FastQC (version 0.11.5)