FastQCFastQC Report
Fri 12 Apr 2019
HJW27AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJW27AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7870100
Sequences flagged as poor quality0
Sequence length71
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5601437.117355560920446No Hit
GGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4003025.086364849239526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATC344500.066.333247
GATCTCT464950.066.197397
TATCTCT448200.066.160747
GGTATTC230000.066.03956612
TCCTTGG254350.065.938927
GATCGTC302200.065.796168
TGATCGT300150.065.722277
TCTCTTG919500.065.720049
AGTCTTG393150.065.632978
CGCATCT366550.065.5953148
GCATCTT367350.065.56249
AACGCAT160450.065.489298
TAAGGTC237550.065.38840513
ACGCATT161250.065.2273569
CGGTTCT321400.065.044588
TAACGCA161300.064.8953557
CTCTTGT929850.064.62456510
GAGTCTT388600.064.596647
TACACGA461750.064.3788762
GTATCTC431650.064.326836
Produced by FastQC (version 0.11.5)