FastQCFastQC Report
Fri 12 Apr 2019
HJW27AFXY_n01_CCPD53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJW27AFXY_n01_CCPD53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11833382
Sequences flagged as poor quality0
Sequence length71
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCG2984300.064.956391
TACACGA4267200.064.934532
ATCGCGC2990800.064.917332
CACGATC4301800.064.8744
TAAGTAG3853050.064.853411
AAGTAGA3861100.064.840322
TAGAGTC3875950.064.7959065
ACGATCT4317550.064.786365
GATCTCT4227300.064.7748957
AGAGTCT3877200.064.745436
ACACGAT4283300.064.743773
GAGTCTT3702850.064.719517
TCTCTTG4280200.064.719189
GCGGTTC3040600.064.695617
GTAGAGT3874150.064.685944
CGATCTC4365650.064.6713946
AGTCTTG3719500.064.6167458
GGTTCTT3068250.064.561139
ATACACG4287300.064.523471
ATCTCTT4290800.064.512228
Produced by FastQC (version 0.11.5)