FastQCFastQC Report
Fri 12 Apr 2019
HJW27AFXY_n01_CCPD52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJW27AFXY_n01_CCPD52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25098996
Sequences flagged as poor quality0
Sequence length71
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGTTTAAGAGCTATGC272580.1086019536398986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATC4464550.064.995017
ACGATCG3630450.064.940131
TCTCTTG5869500.064.868149
AGGTCGC4630750.064.863064
ATACAGG3314800.064.860777
ATAGGTC4606300.064.8579252
GGTCGCA4614650.064.857755
CATCTTG4505100.064.8411810
ACTGTAT6023200.064.837293
TGGTCTT3825750.064.835447
CAGGTAT3296500.064.827510
GCATCTT4510200.064.765769
ATCTCTT5897100.064.761758
GTATCTC6065550.064.755926
TAGGTCG4643450.064.747223
CGATAGG3732250.064.745126
AACGCAT2233950.064.724838
TATCTCT5864000.064.705677
ATAACGC2318550.064.704686
GGTATTC3296200.064.69916512
Produced by FastQC (version 0.11.5)