FastQCFastQC Report
Fri 12 Apr 2019
HJW27AFXY_n01_CCPD51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJW27AFXY_n01_CCPD51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29091731
Sequences flagged as poor quality0
Sequence length71
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATTC5008450.064.947722
TACACGA4776900.064.921972
GATCGCG2815600.064.911661
ATCGTTA5103900.064.8778844
TCGTTAC5115100.064.876975
ATCGCGC2821700.064.850912
TCGATCG5094750.064.84461
CACGATC4819700.064.8377154
ATCGATT5007900.064.8271261
ATGATCG5853100.064.807746
CATGATC5856550.064.8021855
CCATCTT4865100.064.7512811
ATCATGA5656750.064.7488863
TAGAGTC4457600.064.72075
TCCTTGG4814650.064.712177
TCTCTTG4768300.064.696439
GATTCCT5020700.064.692274
CGATCAT5632900.064.6741
TTGGTTC4837500.064.6660910
GATCTCT4724900.064.664367
Produced by FastQC (version 0.11.5)