FastQCFastQC Report
Fri 12 Apr 2019
HJW27AFXY_n01_CCPD50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJW27AFXY_n01_CCPD50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52474917
Sequences flagged as poor quality0
Sequence length71
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCGATCACAAATAGTATGGGTGTTTAAGAGCTATGCTGG596170.11361047031289252No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCGATCACAAATAGTATGGGTGTTTAAGAGCT592610.11293205094540693No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCGATCACAAATAGTATGGGTGTTTAAGAGCTATGCTG556800.10610783815055867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACGA4073000.064.9123462
ACGATCG4729500.064.887411
TCGATTC4276800.064.886322
AGGTCGC4374900.064.853144
GATCGCG2743550.064.8348161
TCGTTAC4463650.064.831065
TCGCATC4232050.064.830317
GGTCGCA4361050.064.819185
ATCGCGC2749400.064.800222
CACGATC4112150.064.778974
ATCGTTA4458550.064.76824
CATGATC4476950.064.761855
ATAACGC2932850.064.743346
TCTCTTG8626550.064.717249
CGATAGG4819300.064.692446
GTATCTC4699700.064.68846
TCGATCG4458950.064.679251
ACTGTAT4673450.064.6691363
ATGATCG4482900.064.63486
ATAGGTC4393700.064.633882
Produced by FastQC (version 0.11.5)