FastQCFastQC Report
Wed 24 Aug 2022
HJVLTDRX2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJVLTDRX2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688244443
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTAT1648954329.76727230962963No Hit
ATCTCTGT1368570388.106470515419312No Hit
TAGCTCTA1345924127.972329632599299No Hit
GGTTCCTC1320133617.819564373356566ABI Solid3 Adapter A (100% over 8bp)
CCTAGACC1276723867.562434843447609No Hit
ATCATGCA1269972117.522442115925271No Hit
CCTGTGCG1224086377.25064652263748No Hit
TAACAAGG1223409257.246635729041757No Hit
GGAGAGAG1054413666.245621979518045No Hit
ATGAGAAA1012410115.996821812136123No Hit
TACTCTTC990948575.869698396513543No Hit
GGACACGT983361655.824758695799836No Hit
ACGTCCCT251994541.4926424964397171No Hit
GAAGGAAC238792201.4144409062923835No Hit
CGCATGTG226384241.3409446774053442No Hit
GGGGGGGG225823331.3376222319957016No Hit
TTTCATGA208613901.2356853941677686No Hit
GGAGCGAG71496030.4234933530890349No Hit
AGGAGAAA24629310.1458871083634895No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)