FastQCFastQC Report
Wed 10 Aug 2022
HJVL2DRX2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJVL2DRX2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2119552327
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACACTC1629901187.689836949234233No Hit
TCGCAATT1503593657.093920875868048No Hit
CATGGCAG1448873946.835754520157218No Hit
AGAACGCC1446739676.825685082508463No Hit
CTGCAGCC1390364196.559706841339992No Hit
CAGTCTGG1376236966.493054889321447No Hit
AAAGCATA1359738876.415217273378502No Hit
TGTAGCGG1323793576.24562815995059No Hit
ATTGGGAA1257872925.934616022338948No Hit
CTTTCGAC1168606185.513457559474539No Hit
GTCTTTGA1136443715.36171575253589No Hit
ACGGGACT1123548215.300875074833668No Hit
TGCATCTG974913394.599619351600938No Hit
GAACATGA969225854.572785666357366No Hit
GCCTTTAT919865394.339904131085884No Hit
GGGGGGGG852354474.0213891355370155No Hit
GGCATACT474863322.240394417023515No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)