FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n02_azbq50k-3.352000000e80b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n02_azbq50k-3.352000000e80b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8097828
Sequences flagged as poor quality0
Sequence length51
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC13700.015.8451487
CCTCTAG14900.015.8260771
CTAGAGG11500.015.3055523
TACCGCC14850.014.97011619
TAGACTA16150.014.4321819
ACCGCCA15650.014.05992220
AATACCG15900.013.9194417
AGGAACC12500.012.7962077
GACCTCG8650.012.67842825
TACACCG11050.012.6189625
TAGGACG10650.012.4490025
GAGACCC9750.012.39580126
TATACAC103900.012.2985145
ATACCGC18550.012.22873718
CCGCCAA18800.012.20549421
GTGTTAG14600.012.1926251
GCGACCT9050.012.01190623
ACCGTCG12100.011.7087529
GGAACCT12750.011.6493578
ATTACGC11400.011.55885921
Produced by FastQC (version 0.11.2)