FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n02_azbq50k-2.352000000e808c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n02_azbq50k-2.352000000e808c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8968305
Sequences flagged as poor quality0
Sequence length51
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGCC16550.017.5215219
TAGACTA17750.017.1194929
CCTCTAG17150.016.5306051
GATAGAC16800.015.7577317
TACACCG12550.015.4136375
GCGACCT11050.015.25890323
CTAGAGG14850.014.8871583
ACCGCCA19200.014.8653420
AATACCG19700.014.70629717
CCGCCAA19950.014.35466821
GACCTCG10450.014.29163925
TGCGACC12350.014.283998522
GGAACCT16550.014.1878788
ACACCGG11400.014.1405986
CGACCTC12200.014.02352824
TTGCGAC12450.013.80123421
TATACAC115650.013.8008445
ACCGTCG13450.013.6925069
AGGAACC16650.013.6875627
CCGTCGT14150.013.44747210
Produced by FastQC (version 0.11.2)