FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n02_azbq50k-1.352000000e8062.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n02_azbq50k-1.352000000e8062.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5139844
Sequences flagged as poor quality0
Sequence length51
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTAG9600.015.5458831
GGAACCT6950.015.1705238
CTAGAGG7600.014.4809023
AGACCGT1951.456101E-714.109746
CGGGAGG4400.014.10274745
TACCACG7200.013.6886027
CCGTGCG1707.776125E-613.4832989
ACCACGG6550.013.2974958
GATAGAC9350.013.2374797
GACCTCG5300.013.05738525
TAGACTA10250.012.7466529
TATACAC65400.012.74114445
AGGAACC8700.012.6457667
CCACGAT8300.012.55097632
ACCGCCA9500.012.52846420
ATACCGA7350.012.4780026
AACCGCG3900.012.3458985
GTCCTAG2104.2450665E-612.2146221
CGTCGTT8150.012.19545711
ACCGTCG7400.012.0803079
Produced by FastQC (version 0.11.2)