FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n02_azbk50r54_abnt-3.352000000e81db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n02_azbk50r54_abnt-3.352000000e81db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8764138
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG12600.014.8744075
TATACAC99100.013.81626345
CTAGAGG13350.012.6690553
TAGACCG4000.012.5706885
GTACTAG14850.012.47322751
CCTCTAG13650.012.0431751
GTCCTAG4350.011.7097811
TTATACA155900.011.36726945
GTGTAAG21400.011.3603281
TACACAG17200.011.2950155
GTCTTAG8700.010.91138651
GTCGTTA10950.010.88693412
GTCTAGG5500.010.5242861
CTTACAC24100.010.4320443
TAGGACG10750.010.417995
GTGTTAG15850.010.3715691
TATACTG23700.010.3188615
ATACCGA14200.010.3012646
TAGACTA14450.010.2781959
GATAGAC13400.010.2305747
Produced by FastQC (version 0.11.2)