FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n02_azbk50r54_abnt-2.352000000e81ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n02_azbk50r54_abnt-2.352000000e81ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10608160
Sequences flagged as poor quality0
Sequence length51
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGG16650.017.5328143
CCTCTAG21050.016.803521
GCGACCT13200.015.8348323
TGCGACC13200.015.58981922
GATAGAC18900.015.075177
TTGCGAC14500.014.98063621
AATACCG22100.014.88511117
CCGCCAA21600.014.820024521
CGACCTC14700.014.667335524
GACCTCG13250.014.6564425
ACCGCCA21600.014.567059520
TACCGCC22550.014.45892819
TAGACTA20600.014.3890919
GGAACCT17750.014.3691548
TAATACG21450.014.038034
CGCCAAA23000.014.01728222
AGGAACC18650.013.922017
GACATCC17650.013.6694548
GGACATC16300.013.5327467
TACACCG14850.013.1569315
Produced by FastQC (version 0.11.2)