FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n02_azbk50r54_abnt-1.352000000e81a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n02_azbk50r54_abnt-1.352000000e81a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10950857
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCT12800.013.97977823
GGGAGAC10900.013.79374924
TGCGACC13250.013.58422822
GAGACCC13050.013.35028626
GGAACCT13400.013.3369958
TACACCG17750.013.3009275
CGACCTC14850.013.14700424
GGAGACC12550.012.99234725
CTAGAGG14850.012.6569893
TTGCGAC14850.012.58088321
GACCTCG13150.012.56939725
GTGTAAG29200.012.3176471
ACCACGG14450.012.2093098
GTACTAG22550.012.1179511
CTTACAC36100.012.0639483
CCTCTAG16850.011.9203341
GTCTAGG4950.011.7957261
CGGGAGG12750.011.6566145
ATCCTAC15500.011.55324642
ACACCGG13950.011.5018286
Produced by FastQC (version 0.11.2)