FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n02_azbk50r54-3.352000000e812e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n02_azbk50r54-3.352000000e812e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13566887
Sequences flagged as poor quality0
Sequence length51
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTAG24450.017.2228641
ACCGCCA26550.016.1363620
GATAGAC24050.015.6621637
TAGACTA27300.015.4709949
TACCGCC27400.015.47032219
CCGCCAA28150.015.3236921
GCGACCT14100.015.24662123
AATACCG27450.015.15360717
CTAGAGG20000.014.9581233
CGACCTC14400.014.05067724
GGAACCT19700.013.4425368
TGCGACC15750.013.26158522
CGCCAAA32900.013.04229722
ACACCGG17350.012.7676136
GACCTCG14650.012.68845725
AGGAACC20550.012.6641457
TATACAC165150.012.6520545
TACACCG20000.012.5602575
CTCTAGA36400.012.5400422
ACCGTCG18850.012.4748429
Produced by FastQC (version 0.11.2)