FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n02_azbk50r54-2.352000000e80f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n02_azbk50r54-2.352000000e80f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7334924
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTAG8350.013.48417951
TAGACAG7250.013.3149795
TACACCG9400.012.959175
CGACCTC7100.012.65134724
GACCTCG6900.012.62318525
GCGACCT7750.012.02976623
TGCGACC8300.011.84534122
CCGGTCG5500.011.69772159
GTATTAG28500.011.5226651
CGACCCC4007.2759576E-1211.4921776
CTGACGC12350.011.11551145
CTAGAGG8950.011.0426053
GTACTAG14100.010.9798031
CTTACAC18300.010.8012373
GTATAGG10050.010.736471
AACCCGG6900.010.659416
GTATATA57750.010.5606611
GGCCTAG2452.2039487E-510.5316481
CTAACAC15200.010.4335383
GGGTAGG4951.8189894E-1210.4252681
Produced by FastQC (version 0.11.2)