FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n02_azbk50r54-1.352000000e80dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n02_azbk50r54-1.352000000e80dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2711419
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGG2503.6379788E-1215.5469176
GCCTAAC2304.105459E-914.1784821
GTACTAG3550.013.7790881
TAGGCCG1503.6772595E-513.7178695
TGGACCG2103.7162135E-713.0646375
ACCGTCG2154.9622577E-712.7571629
GTGCTAC2753.6725396E-912.7053931
TACACCG2907.912604E-1012.6141325
GCTCTAG2703.875175E-812.0779671
TATACTG8700.011.8257485
CTTACAC6250.011.7056913
GGACTAG1608.2945626E-411.646611
TATACAC59350.011.64024345
GTGTAAG6500.011.4674311
ACTGCTC5850.011.3301828
CTAACAC4850.011.313493
ACAGTGC4700.011.184718
TTATACA84200.011.15965445
CCGCGTG1450.00607501211.0342119
TACACAT32600.010.97060745
Produced by FastQC (version 0.11.2)