FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n02_azbabrh-2.352000000e8161.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n02_azbabrh-2.352000000e8161.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9053735
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTAG12450.013.7567361
CTAGGAC4200.012.5865753
TACACCG11350.012.35285
TAGGACG8400.012.0394015
GAGACCC7950.011.23512226
GATAGAC12900.011.2214777
GTGTAAG22900.011.1674221
TAGACTA16900.011.1495239
CTTACAC23250.011.0719483
GGGAGAC7500.011.06809224
TATACAC112200.011.01255845
TAGGACT15150.010.9231734
ACCGCCA15200.010.79642920
CCTATAC11800.010.3234023
CTGACGC13450.010.20742645
TTATACA163300.010.17002445
CTCTAGA19300.010.1022372
TGCGACC8850.010.07711422
TAGACAG9850.010.0337595
CTATACT28700.010.010534
Produced by FastQC (version 0.11.2)