FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n02_azbabrh-1.352000000e8148.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n02_azbabrh-1.352000000e8148.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16033987
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC206970.12908205551120877No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT165470.1031995348380911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTAG22350.012.3400191
GTGTAAG40150.012.166681
TACACCG20400.011.5650525
TATACAC204200.011.21263945
TATACTG37800.011.0285935
CTAGAGG20900.010.9595543
CTACACT26450.010.91148954
CTAGGAC9800.010.7515473
GCGACCT17100.010.72492423
TACACTG27100.010.6498095
CTAGATA32650.010.593344
GTCCTAG9550.010.5238931
GATAGAC20950.010.4926357
CTTACAC41750.010.3691823
TAGACTA25750.010.3154779
TACACAG29150.010.2151645
CCTACAC23400.010.1802083
GTGCTAG16650.010.1071711
TAGGACT22150.010.0308374
TAGACAG17250.09.9589315
Produced by FastQC (version 0.11.2)