FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n02_azb-3.352000000e8049.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n02_azb-3.352000000e8049.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6996555
Sequences flagged as poor quality0
Sequence length51
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTCG7400.017.8653525
CCTCTAG12950.017.3264851
GCGACCT8400.017.23932823
TGCGACC9000.017.1371822
CGACCTC9400.016.26325824
TTGCGAC9100.016.20111521
TACCGCC12350.015.7727119
CTAGAGG10650.015.64283
GGGAGAC6650.015.55115624
CCGTCGT10900.015.4872110
GATAGAC12300.015.3964947
ACCGCCA12450.015.28216120
GATTGCG9400.015.1805519
ACCGTCG10900.015.0721079
CGTCGTT11900.014.95987511
AATACCG13350.014.89856117
TAGACTA13600.014.764269
CCGCCAA13550.014.73045921
GAGACCC8000.014.28429926
GGAGACC7550.013.65218425
Produced by FastQC (version 0.11.2)