FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n02_azb-2.352000000e802f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n02_azb-2.352000000e802f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8163604
Sequences flagged as poor quality0
Sequence length51
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTAG13850.015.900931
CTAGAGG12050.015.8056163
GGAACCT11850.015.2945938
AGGAACC12450.015.1100347
CCGTCGT11600.014.79316410
TACCGCC14950.014.6118619
ACCGCCA15100.014.16531920
ACCGTCG11650.013.9819319
CGACCTC8400.013.91028924
GATAGAC14950.013.8109097
CCACGAT15100.013.6515932
TAGACTA16750.013.5598579
GACCTCG7600.013.52673825
AATACCG16100.013.36956117
ATCCACG15300.013.34844130
CGTCGTT12900.013.3189711
TACACCG11400.013.2852735
CCGCCAA16450.013.18647421
GTCGTTA13650.012.880300512
TCCACGA16250.012.84097431
Produced by FastQC (version 0.11.2)