FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n02_azb-1.352000000e8005.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n02_azb-1.352000000e8005.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6402114
Sequences flagged as poor quality0
Sequence length51
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGG9350.017.6044273
GGAACCT9550.016.5141288
GACATCC8700.014.9749558
AGGAACC10700.014.7390077
CCTCTAG12350.014.3469861
TAGACTA12650.014.0933419
GATAGAC12400.013.8242797
TATACAC92100.013.6500345
GGACATC9500.013.4730927
CCGCCAA14550.013.29680421
ACAGGCG7550.013.23860844
TACCGCC13700.012.99013319
TACACCG8300.012.9457675
ACACCGG6950.012.8291646
TAGGACG7200.012.700935
CACGATG10550.012.63378633
CCACGAT11600.012.4480232
GGGAGAC6700.012.4044424
ATCCACG12050.012.37030330
GCGACCT6050.012.366199523
Produced by FastQC (version 0.11.2)