FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n02_ab-3.352000000e823a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n02_ab-3.352000000e823a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11135343
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTAG16150.013.597531
CTAGAGG15250.013.0196963
TAGGACG15800.012.8553645
TACACCG18050.012.3908275
GGGAGAC10350.012.1786724
GACATCC16700.012.158698
GCGACCT12250.012.01888623
GGACGAG15300.011.9289947
GATAGAC17600.011.9256067
TATACTG32350.011.8518585
GTAGGAC15900.011.6262964
TAGACTA18850.011.6192779
CGACCTC13450.011.379909524
GGACATC16850.011.2378567
TGCGACC13450.011.13996422
ACACCGG14700.011.0229656
GTACTAG20700.010.9437131
TATACAC115400.010.8875745
GACGAGG18100.010.8400968
ACCACGG17000.010.7362928
Produced by FastQC (version 0.11.2)