FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n02_ab-2.352000000e8210.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n02_ab-2.352000000e8210.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13259965
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG20500.013.3251235
GTCTAGG7650.012.5437831
CTTACAC33600.011.7507513
TATACAC150050.011.35289745
CTAGAGG17100.010.739163
GTGTAAG28400.010.3014361
CCTCTAG17000.010.1881
CTAGGAC7300.010.0624473
TAGGACG11650.010.0489895
ACCGTGC13100.09.9843948
GTATTAG49700.09.9364721
TACACAT80800.09.84236345
GTCCTAG7400.09.8047411
TATACTG44500.09.5946925
ACACCGG15850.09.5586056
TTATACA218150.09.53513145
GTACTAG21050.09.3397081
GTATATA84150.09.3174451
GGAACCT16550.09.28955658
TAGGACT22750.09.2828824
Produced by FastQC (version 0.11.2)