FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n02_ab-1.352000000e81f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n02_ab-1.352000000e81f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1921972
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTAG3400.015.7586981
CTAACAC3500.015.6828013
GTCTTAG1207.25445E-515.5303131
TTGCGAC2254.130925E-914.17162221
GTACAGG1801.1854667E-512.9419271
TGCGACC2155.177244E-712.71208722
TATACAC21000.012.48034645
GAGACCC1801.805604E-512.42302426
GATAGAC3152.2191671E-1012.3386327
AGTACCG1300.00267372112.31535155
GTATTAG6250.012.3000081
TACACAG3406.184564E-1112.1083715
AATACCG3751.8189894E-1212.10367217
CCTAGAG2259.414143E-712.09947623
GGGCCTA1701.2563751E-412.03104814
TAGACTA3253.7289283E-1011.9593039
GTACTAG4100.011.9318251
TAGACTG1557.4063614E-411.8045775
CTAGACT1751.5509254E-411.76214
CTAGAGG1953.254949E-511.728593
Produced by FastQC (version 0.11.2)