FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n01_azbq50k-3.351000000e80b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n01_azbq50k-3.351000000e80b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8097828
Sequences flagged as poor quality0
Sequence length51
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTAG15700.019.8602491
GATAGAC16900.017.2960287
TAGACTA17100.017.0937359
CTAGAGG12050.016.6844233
TACCGCC17250.016.03266519
AATACCG17400.015.89533517
TAGGACG9750.015.6817315
ACCGCCA18750.014.51021320
GGAACCT12300.014.0758728
CTAGATA25600.014.0302514
GACCTCG8700.013.9561925
CTCTAGA25350.013.544842
TACACCG10550.013.4269635
AGGAACC13350.013.3056337
ATACCGC21150.013.2889118
CCGCCAA20600.013.20719221
ATCCACG14950.013.08894330
TATACAC117600.012.61989845
CCGTCGT11900.012.48576910
CGCCAAA22550.012.364243522
Produced by FastQC (version 0.11.2)